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+# **Mosquito Pressure Experiment Report**
+
+### **Objective**:
+To evaluate the effects of slow, moderate, and rapid compression rates on *Culex pipiens* mosquitoes using a syringe, with a focus on pressure changes, behavioral observations, and survival rates.
+
+---
+
+## **Experimental Setup**
+
+- **Syringe Size**: 5 mL
+- **Initial Volume**: 5 mL (adjusted for the presence of mosquitoes to ~4.99 mL)
+- **Initial Pressure**: 101.325 kPa (standard atmospheric pressure)
+- **Number of Mosquitoes**: 5
+- **Date**: ____________________
+- **Ambient Temperature**: ____ °C
+- **Ambient Humidity**: ____ %
+
+---
+
+## **Procedure and Compression Rates**
+
+### 1. **Slow Compression**  
+   - **Compression Speed**: 1 mL every 5–10 seconds  
+   - **Goal**: Observe gradual behavior changes and stress without immediate fatalities.  
+
+#### **Boyle’s Law Pressure Calculations**:
+
+| Volume (mL) | Pressure (kPa) |
+|-------------|----------------|
+| 5           | 101.325        |
+| 4           | 126.656        |
+| 3           | 168.875        |
+| 2           | 253.312        |
+| 1           | 505.611        |
+
+#### **Likely Observations**:
+- **At 5 mL (101.325 kPa)**: Baseline; mosquitoes exhibit natural movement.
+- **At 4 mL (126.656 kPa)**: Slight increase in movement, possibly due to mild stress.
+- **At 3 mL (168.875 kPa)**: Disorientation begins, some may slow down or stop flying.
+- **At 2 mL (253.312 kPa)**: Noticeable stress, mosquitoes cling to the walls.
+- **At 1 mL (505.611 kPa)**: Mosquitoes likely immobilized, but most should still survive if decompression is slow.
+
+#### **Survival Prediction**:  
+- **80-100% survival** expected due to gradual pressure changes allowing the mosquitoes time to adjust.
+
+---
+
+### 2. **Moderate Compression**  
+   - **Compression Speed**: 1 mL every 2–3 seconds  
+   - **Goal**: Balance between behavioral observation and potential physical damage.  
+
+#### **Boyle’s Law Pressure Calculations**:
+
+| Volume (mL) | Pressure (kPa) |
+|-------------|----------------|
+| 5           | 101.325        |
+| 4           | 126.656        |
+| 3           | 168.875        |
+| 2           | 253.312        |
+| 1           | 505.611        |
+
+#### **Likely Observations**:
+- **At 4 mL (126.656 kPa)**: Some mosquitoes may stop flying, increased movement erratic.
+- **At 3 mL (168.875 kPa)**: Significant disorientation, mosquitoes cling to surfaces.
+- **At 2 mL (253.312 kPa)**: Labored movement, possible immobilization of a few mosquitoes.
+- **At 1 mL (505.611 kPa)**: Physical stress likely leads to a few fatalities, visible signs of respiratory difficulty.
+
+#### **Survival Prediction**:  
+- **50-70% survival** expected, with some damage to the mosquitoes due to moderate but noticeable pressure changes.
+
+---
+
+### 3. **Rapid Compression**  
+   - **Compression Speed**: From 5 mL to 1 mL in 1–2 seconds  
+   - **Goal**: Test lethal pressure thresholds and observe immediate physical damage.
+
+#### **Boyle’s Law Pressure Calculations**:
+
+| Volume (mL) | Pressure (kPa) |
+|-------------|----------------|
+| 5           | 101.325        |
+| 1           | 505.611        |
+
+#### **Likely Observations**:
+- **At 1 mL (505.611 kPa)**: Immediate trauma to most mosquitoes. Some may suffer ruptured exoskeletons or collapsed tracheal systems, resulting in fatalities.
+
+#### **Survival Prediction**:  
+- **0-20% survival** expected due to the rapid and extreme pressure changes causing immediate damage.
+
+---
+
+## **Summary of Simulations**
+
+| Compression Rate      | Pressure at 1 mL (kPa) | Likely Behavior                                       | Survival Rate (%) |
+|-----------------------|------------------------|------------------------------------------------------|-------------------|
+| **Slow** (5–10 sec)   | 505.611                | Gradual disorientation, some immobilization           | 80-100            |
+| **Moderate** (2–3 sec)| 505.611                | Noticeable disorientation, possible fatalities        | 50-70             |
+| **Rapid** (1–2 sec)   | 505.611                | Immediate trauma, likely fatal                        | 0-20              |
+
+---
+
+## **Further Experimentation: Intermediate Compression Rates**
+
+To fully explore the pressure response of *Culex pipiens* mosquitoes, consider testing compression speeds between the extremes:
+- **Compression over 4, 6, or 8 seconds**: Document whether survival rates improve as compression becomes slower, while still observing behavioral changes.  
+- Reuse the calculations and observations outlined in the **Slow** and **Moderate** compression sections as benchmarks, adjusting based on new findings.
+
+---
+
+### **Detailed Observations and Recording**
+
+When conducting your experiments, use the following structure for recording results:
+
+| Volume (mL) | Time (seconds) | Pressure (kPa) | Behavioral Changes | Visible Damage | Survival Count |
+|-------------|----------------|----------------|--------------------|----------------|----------------|
+| 5           | 0              | 101.325        | Baseline           | None           |                |
+| 4           |                | 126.656        |                    |                |                |
+| 3           |                | 168.875        |                    |                |                |
+| 2           |                | 253.312        |                    |                |                |
+| 1           |                | 505.611        |                    |                |                |
+
+Document each cycle, noting mosquito behavior, visible physical damage, and survival rates. Include a section for **post-experiment examination** (e.g., magnifying glass inspection for exoskeleton damage).
+
+---
+
+### **Next Steps**
+
+- **Analyze results**: Use the survival and behavioral observations to refine the experimental design. 
+- **Follow-up experiments**: Continue testing intermediate compression rates (4, 6, or 8 seconds) to explore survival thresholds.
+- **Report findings**: Record data in a clear, structured format and compare results to the simulations provided.
+
+
+---
+
 ### **Procedure: Mosquito Pressure Experiment**
 
 #### Purpose: