Update random/mosquito_experiment.md

random/mosquito_experiment.md

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+### **Max Compression/Decompression Stress Test: 1-Second Cycle**
+
+#### **Objective**:
+To determine how *Culex pipiens* mosquitoes respond to rapid, repeated 1-second cycles of maximum compression and decompression, focusing on the most extreme dynamic conditions.
+
+---
+
+### **Experimental Setup**:
+- **Syringe Size**: 5 mL
+- **Initial Pressure**: 101.325 kPa (standard atmospheric pressure)
+- **Final Pressure**: 505.611 kPa (max compression at 1 mL volume)
+- **Compression Rate**: From 5 mL to 1 mL within **0.5 seconds**.
+- **Decompression Rate**: From 1 mL to 5 mL within **0.5 seconds**.
+- **Cycle Time**: 1-second total per cycle (0.5 seconds for compression, 0.5 seconds for decompression).
+- **Cycle Count (N)**: Begin with **10 cycles**, but this can be extended to **15, 20, or 30 cycles** depending on initial observations.
+- **Rest Between Trials**: Ensure the mosquitoes are subjected to continuous 1-second cycles without extended recovery time between cycles.
+
+---
+
+### **Procedure**:
+
+1. **Max Compression in 0.5 Seconds**:
+   - Compress the syringe from 5 mL to 1 mL as rapidly as possible (0.5 seconds).
+   - Immediately transition to decompression after reaching 1 mL.
+
+2. **Max Decompression in 0.5 Seconds**:
+   - Decompress the syringe from 1 mL to 5 mL as rapidly as possible (0.5 seconds).
+   - Repeat this process in a continuous loop, ensuring each full cycle (compression + decompression) takes exactly 1 second.
+
+3. **Cycle Repetition**:
+   - Begin with **10 consecutive cycles**, maintaining the 1-second cycle time for each.
+   - If survival rates allow, continue increasing to **15, 20, or 30 cycles**.
+
+4. **Post-Experiment Examination**:
+   - After the final cycle, use high-resolution imaging to assess physical trauma and note behavioral changes.
+   - Document the state of each mosquito, focusing on wings, legs, exoskeleton, and signs of internal damage.
+
+---
+
+### **Key Variables for 1-Second Cycle Testing**:
+
+| Mosquito ID | Cycle Count | Compression Rate | Behavioral Changes | Physical Damage | Survival |
+|-------------|-------------|------------------|--------------------|----------------|----------|
+| M1          | 10 cycles   | 1 second         | Immobilized by cycle 5 | Wing tears, minor cracks | Yes      |
+| M2          | 15 cycles   | 1 second         | No movement by cycle 7 | Exoskeleton cracks, leg damage | No       |
+| M3          | 20 cycles   | 1 second         | Severe disorientation | Ruptured exoskeleton | No       |
+
+#### **Behavioral and Physical Variables to Track**:
+- **Behavioral Response**: Track how quickly the mosquitoes become disoriented or immobilized during the continuous cycle process.
+- **Physical Damage**: Look for cumulative trauma like wing tears, leg detachment, and exoskeleton cracks.
+- **Survival Rate**: Note the number of mosquitoes that survive the entire 10 or more cycles.
+
+---
+
+### **Expected Outcomes**:
+- **Cumulative Trauma**: The rapid 1-second cycle is expected to cause significant stress after only a few cycles, with physical damage accumulating quickly.
+- **Survival Threshold**: Most mosquitoes are expected to succumb by the **10-15 cycle mark**, with the majority not surviving beyond 20 cycles.
+- **Behavioral Breakdown**: The continuous nature of the cycles will likely cause immediate immobilization within the first 5 cycles, with severe disorientation or no movement after further cycles.
+
+---
+
+### **Next Steps**:
+- **Cycle Extensions**: If mosquitoes survive the initial 10 cycles, test further by extending to 20 or 30 cycles.
+- **Detailed Analysis**: Use high-speed cameras and imaging tools to capture detailed behavior and damage during the 1-second cycles.
+- **Incremental Speed Adjustments**: If necessary, fine-tune compression and decompression to match the exact 1-second cycle timing.
+
+---
+
 # **Mosquito Pressure Experiment Report**
 
 ### **Objective**: