Update random/mosquito_experiment.md

random/mosquito_experiment.md

@@ -60,4 +60,82 @@ To observe the effects of rapid air pressure changes on *Culex pipiens* (common
 #### **Documentation**:
 - Record the **starting air volume** and the **compressed volume** for each phase.
 - Note any **visible physical damage** to the mosquitoes after each pressure change.
-- Provide a summary of the mosquitoes’ behavior throughout the experiment.
+- Provide a summary of the mosquitoes’ behavior throughout the experiment.
+
+---
+
+# Mosquito Pressure Experiment: Detailed Data Collection Sheet
+
+## Experimental Setup
+- Syringe size: ____ mL
+- Number of mosquitoes: ____
+- Initial air volume: ____ mL
+- Date and time: ____________________
+- Ambient temperature: ____ °C
+- Ambient humidity: ____ %
+
+## Observation Table
+
+| Phase | Cycle | Volume (mL) | Estimated Pressure (kPa) | Behavior Changes | Visible Damage | Survival Count |
+|-------|-------|-------------|--------------------------|-------------------|----------------|----------------|
+| Initial | - | | 101.325 (atmospheric) | | | |
+| Compression | 1 | | | | | |
+| Decompression | 1 | | | | | |
+| Compression | 2 | | | | | |
+| Decompression | 2 | | | | | |
+| Compression | 3 | | | | | |
+| Decompression | 3 | | | | | |
+
+## Detailed Observations
+
+### Initial Conditions
+Describe the mosquitoes' behavior and appearance before the experiment:
+_______________________________________________________________________
+_______________________________________________________________________
+
+### Compression Phase Observations
+Note any changes in movement, appearance, or visible damage during compression:
+_______________________________________________________________________
+_______________________________________________________________________
+
+### Decompression Phase Observations
+Describe signs of stress, disorientation, or physical damage after rapid decompression:
+_______________________________________________________________________
+_______________________________________________________________________
+
+### Cumulative Effects
+Document any observed differences in mosquito reaction with repeated cycles:
+_______________________________________________________________________
+_______________________________________________________________________
+
+## Post-Experiment Examination
+
+### Physical Damage Assessment
+Use a magnifying glass or microscope to inspect and describe any damage:
+
+1. Exoskeleton condition: ________________________________________________
+2. Legs and wings: ______________________________________________________
+3. Visible internal structure changes: ______________________________________
+
+### Survival Rate
+- Number of mosquitoes at start: ____
+- Number of mosquitoes surviving at end: ____
+- Survival rate: _____%
+
+## Additional Notes
+_______________________________________________________________________
+_______________________________________________________________________
+_______________________________________________________________________
+
+## Calculations
+
+1. Estimated Pressure (using Boyle's Law):
+   P2 = (P1 * V1) / V2
+   Where:
+   - P1 = Initial pressure (101.325 kPa)
+   - V1 = Initial volume (mL)
+   - V2 = New volume (mL)
+   - P2 = New pressure to be calculated (kPa)
+
+2. Survival Rate:
+   Survival Rate (%) = (Number of surviving mosquitoes / Initial number of mosquitoes) * 100